VB.Net读取或保存listbox的记录到文件中的代码如下：

Public Class Form1
    Inherits System.Windows.Forms.Form

#Region " Windows Form Designer generated code "

    Public Sub New()
        MyBase.New()

        'This call is required by the Windows Form Designer.
        InitializeComponent()

        'Add any initialization after the InitializeComponent() call

    End Sub

    'Form overrides dispose to clean up the component list.
    Protected Overloads Overrides Sub Dispose(ByVal disposing As Boolean)
        If disposing Then
            If Not (components Is Nothing) Then
                components.Dispose()
            End If
        End If
        MyBase.Dispose(disposing)
    End Sub

    'Required by the Windows Form Designer
    Private components As System.ComponentModel.IContainer

    'NOTE: The following procedure is required by the Windows Form Designer
    'It can be modified using the Windows Form Designer. 
    'Do not modify it using the code editor.
    Friend WithEvents ListBox1 As System.Windows.Forms.ListBox
    Friend WithEvents Button1 As System.Windows.Forms.Button
    Friend WithEvents ListBox2 As System.Windows.Forms.ListBox
    Friend WithEvents Button2 As System.Windows.Forms.Button
    <System.Diagnostics.DebuggerStepThrough()> Private Sub InitializeComponent()
        Me.ListBox1 = New System.Windows.Forms.ListBox()
        Me.Button1 = New System.Windows.Forms.Button()
        Me.ListBox2 = New System.Windows.Forms.ListBox()
        Me.Button2 = New System.Windows.Forms.Button()
        Me.SuspendLayout()
        '
        'ListBox1
        '
        Me.ListBox1.Location = New System.Drawing.Point(72, 32)
        Me.ListBox1.Name = "ListBox1"
        Me.ListBox1.SelectionMode = System.Windows.Forms.SelectionMode.MultiSimple
        Me.ListBox1.Size = New System.Drawing.Size(184, 69)
        Me.ListBox1.TabIndex = 2
        '
        'Button1  '更多vb.net源码和实例，请关注{乐博网 www.lob.cn}
        '
        Me.Button1.Location = New System.Drawing.Point(128, 112)
        Me.Button1.Name = "Button1"
        Me.Button1.Size = New System.Drawing.Size(72, 24)
        Me.Button1.TabIndex = 3
        Me.Button1.Text = "Write"
        '
        'ListBox2
        '
        Me.ListBox2.Location = New System.Drawing.Point(72, 160)
        Me.ListBox2.Name = "ListBox2"
        Me.ListBox2.Size = New System.Drawing.Size(184, 69)
        Me.ListBox2.TabIndex = 4
        '
        'Button2
        '
        Me.Button2.Location = New System.Drawing.Point(136, 248)
        Me.Button2.Name = "Button2"
        Me.Button2.Size = New System.Drawing.Size(72, 24)
        Me.Button2.TabIndex = 5
        Me.Button2.Text = "Load"
        '
        'Form1
        '
        Me.AutoScaleBaseSize = New System.Drawing.Size(5, 13)
        Me.ClientSize = New System.Drawing.Size(320, 293)
        Me.Controls.AddRange(New System.Windows.Forms.Control() {Me.Button2, Me.ListBox2, Me.Button1, Me.ListBox1})
        Me.Name = "Form1"
        Me.StartPosition = System.Windows.Forms.FormStartPosition.CenterScreen
        Me.Text = "Form1"
        Me.ResumeLayout(False)

    End Sub

#End Region
    Dim w As IO.StreamWriter
    Dim r As IO.StreamReader

    Private Sub Form1_Load(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles MyBase.Load
        Dim itms() As String = {"item1", "item2", "item3"}
        ListBox1.Items.AddRange(itms)
    End Sub

    Private Sub Button1_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button1.Click
        Dim i As Integer
        w = New IO.StreamWriter("c:\test.txt")
        For i = 0 To ListBox1.Items.Count - 1
            w.WriteLine(ListBox1.Items.Item(i))
        Next
        w.Close()
    End Sub

    Private Sub Button2_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button2.Click
        r = New IO.StreamReader("c:\test.txt")
        While (r.Peek() > -1)
            ListBox2.Items.Add(r.ReadLine)
        End While
        r.Close()
    End Sub
End Class